Journal: European Journal of Medical Research
Article Title: Mechanism of ultrasound-guided renal parenchymal injection of MSCs for the treatment of chronic kidney disease: down-regulated SGK1 promotes M2 macrophage polarization
doi: 10.1186/s40001-025-03724-8
Figure Lengend Snippet: A – F THP-1-derived macrophages were transfected with blank overexpression vectors (Co-culture + OE-NC) or SGK1 overexpression vectors (Co-culture + OE-SGK1), and co-cultured with MSCs for 12 h: Fluorescence intensity analysis of CD68, iNOS, and CD163 in macrophages. G–J The supernatant levels of pro-inflammatory cytokines IL-1β, IL-10, MCP-1, and TNF-α in cell supernatant. ns p > 0.05, ** p < 0.01, *** p < 0.001
Article Snippet: Human umbilical cord MSCs (MEISEN, CTCC-159-HUM), human acute monocytic leukemia cell line THP-1 (MEISEN, CTCC-001-0044) and human renal tubular epithelial cell line HK-2 cells (ATCC, CRL-2190) were cultured in DMEM (Gibco, 11,965–092) with 10% fetal bovine serum (FBS; Gibco, 10,099–141) and 1% penicillin–streptomycin (Gibco, 15,140-122).
Techniques: Derivative Assay, Transfection, Over Expression, Co-Culture Assay, Cell Culture, Fluorescence